Tag: Science Fail

Science Fail Monday: How a dead salmon taught us about statistics

Science Fail Monday: How a dead salmon taught us about statistics

Any scientist knows the importance of a good negative control. A negative control in an experiment is a group of samples or subjects in which no response is expected to an experimental treatment. The experimental group can then be compared to the control group. Such…

Statistically significant insults for the soul: p < 0.05

Statistically significant insults for the soul: p < 0.05

Author’s forward There are days in which a scientist finds herself needing to express her “mean genes.” When an experimental control fails, when Reviewer 3 sinks a paper, when someone drinks the last cup of communal coffee and fails to make more, the time has…

Science Fail Monday: Requiem for a Western Blot

Requiem for a Western Blot: A Haiku on Reversing the Positive and Negative Electrodes Two weeks to prepare It’s time to transfer this gel Data finally? Excitement building Put the wires on backwards Proteins all lost, $%!#   PEER EDITED BY Bailey Peck AND chris givens Follow…

Inaugural Science Fail Monday: Filter Fail

For weeks, I struggled to produce a successful harvest of lentivirus. I needed to transduce my pancreatic cancer cell lines with shRNA targeting my gene of interest. This is a common protocol in my lab, and I had never experienced difficulty accomplishing this task before.…