Any scientist knows the importance of a good negative control. A negative control in an experiment is a group of samples or subjects in which no response is expected to…
Continue ReadingAuthor’s forward There are days in which a scientist finds herself needing to express her “mean genes.” When an experimental control fails, when Reviewer 3 sinks a paper, when someone…
Continue ReadingRequiem for a Western Blot: A Haiku on Reversing the Positive and Negative Electrodes Two weeks to prepare It’s time to transfer this gel Data finally? Excitement building Put the…
Continue ReadingFor weeks, I struggled to produce a successful harvest of lentivirus. I needed to transduce my pancreatic cancer cell lines with shRNA targeting my gene of interest. This is a…
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